Stang, have you roughed out a plan for your basement? It can be helpful to use some chalk and draw lines on your floor to see what might work.
When framing, remember to use pressure treated wood for your bottom plates (the wood touching the cement floor). I have not used a subfloor, so I have no option on that, but as I recall from shows on tv, it seems they were laying the subfloor first, which makes sense as it would be easier to not have to cut around anything. And, I suppose you could screw your bottom plate right into the subfloor.
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