Separation is one of these heartstring footballs that wins elections but ultimately distracts from more pressing issues such as the economy.
The Supreme Court Reference re Secession of Quebec and subsequent legal and political developments have basically watered down the significance of another referendum in Quebec.
In 1995 no one knew what would happen if Quebeckers had voted for sovereignty. Today we know that the path to independence is strewn with impediments, and any attempt to avoid them altogether is likely to be viewed by the international community as an act of bad faith. Unless the National Assembly of Quebec plays by the ground rules set down since 1995 and can successfully navigate the minefield (meaning the Government of Canada would have to grant its blessing for secession) then Quebec is unlikely to ever be an independent nation in its own right.
There's a good overview of subsequent developments here:
http://en.wikipedia.org/wiki/Clarity_Act